n acute liver failure (ALF), a poorly controlled innate immune response causes massive hepatic destruction , which elicits a systemic inflammatory response, progressive multiple organ failure and ultimate sudden death.
Although the liver has inherent tissue-reparing activities, its regeneration during ALF fails, and orthotopic liver transplantation is the only curative approach. We show that a single intravenous administration of the stem cells from human exfoliated deciduous teeth derived conditioned medium (SHEDCM) into the ALF mice markedly improved the condition of injured liver and animal’s survival rate.
Representative images of H&E – stained liver: in vacuolization was observed through the PBS- or DMEM-treated control liver ; in the Fibro-CM and BMSC-CM groups, the tissue destruction was modest compared with the control, but severe pan-loular hepatocellular death was observed; a single injection of SHEDCM restored the normal liver structure.
filtration of mononuclear immune cells was evident before treatment; severe hepatocellular death with cytoplasmatic Also both SHED and SHEDCM exrted similar levels of therapeutic effect, suggesting that the SHED reversed ALF by paracrine mechanisms. Importantly , SHEDCM attenuated the ALF-induced pro-inflammatory response and generated an anti-inflammatory/tissue regeneration environment.
(Matsushita,Y.et al: Multifaced therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for acute failure in rats. Tissue Engineering. Regen.Med.2015 Published online in widely Online Libraly DPI:101002/term.2086)